Breakdancer software download

Editorial video. Breakdancer - stock photo Female Hip-hop dancer breakdancing, standing on one hand. About 25 years old Caucasian brunette. Contact your company to license this image. All Royalty-Free licenses include global use rights, comprehensive protection, simple pricing with volume discounts available. Newspapers and magazines except for covers , editorial broadcasts, documentaries, non-commercial websites, blogs and social media posts illustrating matters of public interest.

Book or magazine covers, commercial, promotional, advertorial, endorsement, advertising, or merchandising purposes in any media e. Anyone in your organization can use it an unlimited number of times for up to 15 years, worldwide, with uncapped indemnification. Protect your creative work - we'll remove this image from our site for as long as you need it. Approvals and clearances are based on the intended use.

Please contact us to tell us about your project or request a preview. Easy-access agreement. Images marked as Easy-access downloads are not included in your Premium Access or subscription package with Getty Images, and you will be billed for any images that you use. A classic, this just put you in the middle of it. It was a half drama and half documentary a docudrama?

PG 90 min Comedy, Drama, Music. A struggling young jazz dancer meets up with two break-dancers. Together they become the sensation of the street crowds. Amazing how they dug up the early California hip hop scene, pop locking, sliding, Ice T and all and gave a fascinating look into what was going down in La La land.

PG 94 min Comedy, Drama, Musical. A developer tries to bulldoze a community recreation center. The local breakdancers try to stop it. PG 84 min Drama, Music. An aspiring DJ, from the South Bronx, and his best friend, a promoter, try to get into show business by exposing people to hip-hop music and culture.

Not sure why, but though I swear by this movie, it just seemed so dreary and dramatic besides the scenes where artists performed to really evoke the nature of the culture. Great performances, but a depressing plot.

R 91 min Comedy, Sport. A gang of boys under the Brooklyn Bridge are united by their common interest in break dancing. Some work as pizza delivery boys, hence they call themselves the "Delivery Boys".

They form a See full summary ». Votes: This movie just epitomized hip hop. There was lots and lots of break dancing, some good, some OK, some so-so. Was light in the music too, but these were some down kids! A young breakdancer experiences a series of complications involving a poor opera performer he befriends, his older sister and jealous cousin, a female student, two rivals, and a lunatic. This I found out about on a blog. Its a Chinese? I have not seen it, but will look for it.

PG min Drama, Musical. Eight small-town teens travel to New York City for a one-in-a-million shot at stardom in a national dance competition. Affymetrix SNP6. Reference alleles for the SNP array data were retrieved from dbSNP, and genotype calls that either did not match with dbSNP or were homozygous for the reference allele were removed, leaving , out of , variant loci. High impact variants with an ExAC minor allele frequency 0.

The list of genes that contained a rare, high impact variant were added to the list of genes with exons that overlap a homozygous deletion, and this deleterious gene set was used to probe for biological processes that might be altered by genomic variants. Gene set enrichment was determined using a hypergeometric test of the deleterious gene set against the MSigDB version 5.

MSigDB gene sets with fewer than 50 genes were not considered. Yearly publication counts for PubMed queries were downloaded as comma-separated tables and plotted in Fig. Supplementary Materials. Supplementary figures S1 to S8 and captions for supplementary tables S1 and S2. PDF kb. Supplementary Table S1. Long deletions found in Jurkat with matches to pathogenic variants in dbVar.

All database matches are included with one dbVar entry per row. CSV 7 kb. Supplementary Table S2. Duplications found in Jurkat with matches to pathogenic variants in dbVar.

CSV 17 kb. We dedicate this work to Dr. Daniel R. Salomon, who conceived this study but passed away prior to its completion. DRS conceived the study and supplied the samples. LG conceptualized and performed the analyses and drafted the manuscript. AS performed the sequencing. SRH supervised the sequencing and suggested revisions to the manuscript.

AIS supervised the data analysis and suggested revisions to the manuscript. All authors have read and approved the manuscript. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Electronic supplementary material.

Louis Gioia, Email: ude. Azeem Siddique, Email: ude. Steven R. Head, Email: ude. Andrew I. Su, Email: ude. National Center for Biotechnology Information , U. BMC Genomics. Published online May 8. Head , 2 Daniel R. Salomon , 1 and Andrew I. Author information Article notes Copyright and License information Disclaimer.

Corresponding author. Received May 30; Accepted Apr This article has been cited by other articles in PMC. Additional file 2: Supplementary Table S1. Additional file 3: Supplementary Table S2.

Abstract Background The Jurkat cell line has an extensive history as a model of T cell signaling. Results Here, we report a comprehensive catolog of genomic variation in the Jurkat cell line based on whole-genome sequencing.

Electronic supplementary material The online version of this article Background The Jurkat cell line was isolated in from the blood of a fourteen-year-old boy with Acute Lymphoblastic Leukemia [ 1 ]. Open in a separate window. Results Sequencing and variant callers Whole-genome sequencing of the Jurkat cell line produced over million bp paired-end reads and over million bp paired-end reads, totaling over billion sequenced bases.

Table 1 Variant loci counts from each tool. Translocation Inter. The percentage of sites that overlap the other tools is provided where applicable. Comparisons to databases After creating the merged variant sets, we compared them to databases of previously identified variants in order to assess the novelty of the genomic variants that were detected in Jurkat. Table 2 Jurkat variants found in the ClinVar database. Table 3 Gene sets enriched for highly impacted genes.

Defective pathways By leveraging the deep history of the Jurkat cell line, in combination with our pathogenic and high-impact variant lists, we have distinguished three core pathways that are defective due to genomic aberrations in Jurkat—namely TCR signaling, genome stability, and O-linked glycosylation. Discussion We performed a bottom-up search for abnormalities in the Jurkat genome using short-read sequencing. Conclusions Using whole-genome sequencing, we created a comprehensive catalog of genomic variants in the Jurkat cell line.

Data preprocessing Data quality was checked using the FastQC software version 0. Variant caller comparisons Structural variant calls from each tool were separated by class for further analyses. Variant call merging Given the differences in breakpoint detection precision between the individual variant callers, the variant calls were merged hierarchically, with the more precise, split-read calls of GATK and Pindel taking precedence over the less precise variant calls from BreakDancer and CNVnator.

Database comparisons Small variant functional predictions were annotated with the SnpEff software package version 4. Publication trends Yearly publication counts for PubMed queries were downloaded as comma-separated tables and plotted in Fig. Additional files Additional file 1 2. Additional file 2 6. Additional file 3 17K, csv Supplementary Table S2. Acknowledgements We dedicate this work to Dr. Notes Ethics approval and consent to participate Not applicable.

Competing interests The authors declare that they have no competing interests. Footnotes Electronic supplementary material The online version of this article Contributor Information Louis Gioia, Email: ude. References 1. Characterization of ebv-genome negative null and t cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-hodgkin lymphoma.

Int J Cancer. Abraham RT, Weiss A. Jurkat t cells and development of the t-cell receptor signalling paradigm. Nat Rev Immunol. Pi 3-k and t-cell activation: limitations of t-leukemic cell lines as signaling models. Trends Immunol. Aligning sequence reads, clone sequences and assembly contigs with bwa-mem. The genome analysis toolkit: a mapreduce framework for analyzing next-generation dna sequencing data.

Genome Res. Pindel: a pattern growth approach to detect break points of large deletions and medium sized insertions from paired-end short reads. Breakdancer: an algorithm for high-resolution mapping of genomic structural variation. Nat Methods. Cnvnator: an approach to discover, genotype, and characterize typical and atypical cnvs from family and population genome sequencing.

Snow K, Judd W. Heterogeneity of a human t-lymphoblastoid cell line. Exp Cell Res. Cheng J, Haas M. Frequent mutations in the p53 tumor suppressor gene in human leukemia t-cell lines. Mol Cell Biol. Integrated view of genome structure and sequence of a single dna molecule in a nanofluidic device.

Proc Natl Acad Sci. The characterization of twenty sequenced human genomes. PLoS Genet. The diploid genome sequence of an individual human. PLoS Biol. Dbvar and dgva: public archives for genomic structural variation. Nucleic Acids Res. The database of genomic variants: a curated collection of structural variation in the human genome.

Analysis of protein-coding genetic variation in 60, humans. Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Deficiency of pten in jurkat t cells causes constitutive localization of itk to the plasma membrane and hyperresponsiveness to cd3 stimulation. Pten gene alterations in lymphoid neoplasms. Inactivation of ship1 in t-cell acute lymphoblastic leukemia due to mutation and extensive alternative splicing.

Leuk Res. Characterization of ctla-4 structure and expression on human t cells. J Immunol. Ctla-4 is not restricted to the lymphoid cell lineage and can function as a target molecule for apoptosis induction of leukemic cells.